AJSM signin
HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Sign In to gain access to subscriptions and/or personal tools.
First published on April 16, 2004, doi:10.1177/0363546503261695
This version was published on June 1, 2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
32/4/870    most recent
0363546503261695v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yasuda, K.
Right arrow Articles by Tohyama, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yasuda, K.
Right arrow Articles by Tohyama, H.
Related Collections
Right arrow Reconstruction
Right arrow Animal studies
Right arrow Biomechanics
The American Journal of Sports Medicine 32:870-880 (2004)
© 2004 American Orthopaedic Society for Sports Medicine

The Effect of Growth Factors on Biomechanical Properties of the Bone–Patellar Tendon–Bone Graft After Anterior Cruciate Ligament Reconstruction

A Canine Model Study

Kazunori Yasuda, MD, PhD*,{dagger},{ddagger}, Fumihisa Tomita, MD, PhD{ddagger}, Shuji Yamazaki, MD, PhD{ddagger}, Akio Minami, MD, PhD{ddagger} and Harukazu Tohyama, MD, PhD{dagger}

From the Department of Sports Medicine and Joint Reconstruction Surgery, and the {ddagger} Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Sapporo, Japan

* Address correspondence to Kazunori Yasuda, MD, PhD, Department of Sports Medicine and Joint Reconstruction Surgery, Hokkaido University School of Medicine, Kita-ku, Kita-15, Nishi-7, Sapporo, 060-8638, Japan.

Background: No studies have dealt with the effect of growth factors on the free tendon autograft in anterior cruciate ligament reconstruction.

Hypothesis: Application of exogenous transforming growth factor–ß and epidermal growth factor may affect the structural properties and histology of the bone–patellar tendon–bone autograft after anterior cruciate ligament reconstruction.

Study Design: Controlled laboratory study.

Methods: Twenty dogs underwent anterior cruciate ligament reconstruction with the autogenous bone–patellar tendon–bone graft in bilateral knees. In 10 animals, 12 ng transforming growth factor–ß and 300 ng epidermal growth factor mixed with fibrin sealant of 0.6 mL were applied to the left knee. In the remaining 10 dogs, fibrin sealant alone was applied to the left knee. No additional treatments were applied to the right knee.

Results: The growth factor application increased the stiffness and maximum failure load of the femur-graft-tibia complex at 12 weeks (P = .016 and P = .012, respectively); the sham treatment did not significantly affect them. Histologically, most of the cells in the grafts treated with growth factors had spindle-shaped nuclei; cells in the other grafts had round-shaped nuclei.

Conclusions: Application of transforming growth factor–ß and epidermal growth factor improves the structural properties of the autograft after anterior cruciate ligament reconstruction in the canine model.

Clinical Relevance: Application of growth factors is a possible strategy to prevent graft deterioration in anterior cruciate ligament reconstruction.

Key Words: anterior cruciate ligament (ACL) reconstruction • bone-tendon-bone autograft • transforming growth factor ß (TGF-ß) • biomechanics • growth factor




This article has been cited by other articles:


Home page
 Am J Sports MedHome page
M. Y. H. Soon, A. Hassan, J. H. P. Hui, J. C. H. Goh, and E. H. Lee
An Analysis of Soft Tissue Allograft Anterior Cruciate Ligament Reconstruction in a Rabbit Model: A Short-Term Study of the Use of Mesenchymal Stem Cells to Enhance Tendon Osteointegration
Am. J. Sports Med., June 1, 2007; 35(6): 962 - 971.
[Abstract] [Full Text] [PDF]

Home page
 Am J Sports MedHome page
M. Kobayashi, N. Watanabe, Y. Oshima, Y. Kajikawa, M. Kawata, and T. Kubo
The Fate of Host and Graft Cells in Early Healing of Bone Tunnel After Tendon Graft
Am. J. Sports Med., December 1, 2005; 33(12): 1892 - 1897.
[Abstract] [Full Text] [PDF]

Home page
 Am J Sports MedHome page
B. D. Beynnon, R. J. Johnson, J. A. Abate, B. C. Fleming, and C. E. Nichols
Treatment of Anterior Cruciate Ligament Injuries, Part 2
Am. J. Sports Med., November 1, 2005; 33(11): 1751 - 1767.
[Abstract] [Full Text] [PDF]

Home page
 Am J Sports MedHome page
E. Kondo, K. Yasuda, M. Yamanaka, A. Minami, and H. Tohyama
Effects of Administration of Exogenous Growth Factors on Biomechanical Properties of the Elongation-type Anterior Cruciate Ligament Injury With Partial Laceration
Am. J. Sports Med., February 1, 2005; 33(2): 188 - 196.
[Abstract] [Full Text] [PDF]


HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Orthopaedic Society for Sports Medicine.